Post Date: July 12, 2022
Post-doctoral Position in diatoms plastid biogenesis and function at IBPC, Paris, France
A two-year position is open in the laboratory of “Chloroplast Biology and Light Sensing in Microalgae” at IBPC, in the center of Paris, France. This CNRS research institute groups several laboratories active in microalgal biology, microbiology, membrane biology, structural biology and structural modeling. The host laboratory has a long-lasting expertise in photosynthesis, chloroplast biology and microalgal research. In the framework of the IBPC-affiliated Laboratory of Excellence (Labex) Dynamo, the project is funded to study the biogenesis of the photosynthetic apparatus and the crosstalk between nuclear and plastid genomes in diatoms.
Diatoms, a group of more than 100 000 different phytoplanktonic species, are among the most successful photosynthetic eukaryotic microalgae and colonize all aquatic zones (oceans, intertidal zones, freshwater bodies, soil, and ice zones). They are responsible of about 20% of the global carbon fixation and oxygen production on earth. They arose from a secondary endosymbiosis between a heterotrophic protist and, at least, one other photosynthetic eukaryote from the red lineage that itself arose from a primary endosymbiosis between a heterotrophic protist and a cyanobacterium. However, despite their ecological importance as primary producers and a growing biotechnological interest, our current understanding of the regulation of diatom photosynthesis is still limited and even the most basic questions about plastid biogenesis remain unanswered. Furthermore, genetic approaches to study plastid functions in diatoms remain so far limited because the most studied diatom models are obligate phototrophs, in which photosynthesis and plastid biogenesis are essential for survival.
To fill this important knowledge gap, the laboratory has selected the centric diatom Cyclotella cryptica as a novel model system for the study of diatoms plastid biology, since it is the only diatom that combine the availability of genomic and genetic resources with the ability to grow heterotrophically in the dark, using glucose as a source of reduced carbon, thus potentially allowing the isolation of photosynthetic mutants. The recruited post-doctoral fellow will use the tools already developed in the laboratory (biolistic transformation of the chloroplast - homologous recombination - and nuclear - CRISPR/cas9 - compartments of C. cryptica,) to construct photosynthesis mutants in order to characterise the photosynthetic apparatus of diatoms, and the biogenesis of the plastid.
Recent references from the lab related to the project:
Lepetit B, Campbell D A, Lavaud J, Büchel C, Goss R & Bailleul B (2022) Photosynthetic Light Reactions in Diatoms. II. The Dynamic Regulation of the Various Light Reactions. pp 423-464, In: Comprehensive overview on diatom molecular biology, 1st Edition 2022, A. Falciatore and T Mock Eds, Springer International Publishing 978-3-030-92499-7 (ISBN)
Jaubert M, Duchêne C, Kroth P G, Rogato A, Bouly J-P & Falciatore A (2022) Sensing and Signalling in Diatom Responses to Abiotic Cues. pp 607-639, In: Comprehensive overview on diatom molecular biology, 1st Edition 2022, A. Falciatore and T Mock Eds, Springer International Publishing 978-3-030-92499-7 (ISBN)
Blommaert L, Chafai L, Bailleul B. The fine-tuning of NPQ in diatoms relies on the regulation of both xanthophyll cycle enzymes - 2021 - Sci Rep. 11(1):12750 - https://doi.org/10.1038/s41598-021-91483-x
Falciatore A, Jaubert M, Bouly J-P, Bailleul B, Mock T. Diatom Molecular Research Comes of Age: Model Species for Studying Phytoplankton Biology and Diversity - 2020 - Plant Cell, 32(3):547-572 - https://doi.org/10.1105/tpc.19.00158
Annunziata R, Ritter A, Fortunato AE, Manzotti A, Cheminant-Navarro S, Agier N, Huysman M, Wing P, Bones A, Bouget F-Y, Cosentino Lagomarsino M, Bouly JP and Falciatore A (2019) bHLH-PAS protein RITMO1 regulates diel biological rhythms in the marine diatom Phaeodactylum tricornutum, PNAS, 116(26): 13137-13142 - doi 10.1073/pnas.1819660116
Choquet Y. andWollman F.-A. (2022) The Assembly of Photosynthetic Proteins, In: The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use 3rd Edition, Vol. 2: Organellar and metabolic processes, F.-A. Wollman and A R Grossman Eds., Elsevier (Amsterdam), in press.
Required skills: applicants should be qualified in molecular biology and microbiology and have a strong interest for organelle biology and regulation of gene expression. Previous experience with microalgae or functional studies of photosynthesis would be an asset. The candidate must be innovative, rigorous, and have good communication skills to work in a team.
Restriction of applications: the funding agency requires that applicants should not have worked in France for more than twelve months within the last three years.
Funding: coFund Fellowship Program associated to the DynaMO labex, funded by the European Union’s Horizon 2020 Research and Innovation Program under the Marie Skłodowska-Curie actions.
Supervisor: Angela Falciatore, and Yves Choquet
Laboratory of Chloroplast Biology and Light-sensing in Microalgae, headed by Dr. Angela Falciatore, and adjunct director Dr. Yves Choquet.
Duration: 24 months, flexible start between November 2022 and January 2023.
Applications will be open during summer. Please refer to the Labex Dynamo website to apply: http://labexdynamo.ibpc.fr/fp-dynamo-paris/